FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945156
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAG8801.2286723E-49.0034411
CTCACGA11603.5320463E-68.6929781
CTCTGAC15903.1383734E-56.795051
GTCCAAT13852.969934E-46.7607071
GTTTAAT22706.681148E-86.6633391
CTTCGAT33859.640644E-116.1707481
CTCAGCT13250.0094816135.9796441
CCCACAG16350.00219993855.72695971
CTTGGAT21706.228993E-55.64270971
CCCAAAT19454.982794E-45.5548221
CCACGAT25652.4685814E-55.33537241
CTCCAAT24858.515913E-55.2172861
CCCGTTT29307.685969E-65.16238261
TTCGATT30803.700321E-65.1285642
GTTCGAT25401.20137476E-45.10431341
CCCAGAT27055.948604E-55.0592351
CGTAAAT27206.515069E-55.03133441