FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014735
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT13452.3637567E-68.1612471
CTCGCGA9050.00137259088.0860981
CTCTAAA21602.4374458E-97.45343541
CTCCTAA15151.2775616E-57.24546341
CTTCAAT53800.07.0730811
CCCACAT19952.5963527E-76.9694461
CTCACAA15902.515204E-56.90369651
CCTCGAG11950.00274142446.7361591
CTCCAAT26252.4938345E-96.6906681
CTCGAAC13150.00101709176.67794851
CTCACGA16454.0402378E-56.6728741
CCCTCTC12100.00312501156.65265271
CTTAAAG32852.910383E-116.46026231
CCCACGA13750.00168476.38654661
CTCAAAC27557.046765E-96.37495661
CCCAGAG12650.0049739076.3634071
CTTCGAA20753.654348E-66.3480741
CTTAACT18703.5112436E-56.26132061
CCCATTA14050.00214791276.2501791
CTCCAAG24602.2629138E-76.2470031
Produced by FastQC (version 0.11.5)