FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113087
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGT4600.006447312410.9454211
CTCACGG6053.7286984E-410.6998921
AGTCAAT7550.0028300688.5740851
CCCAGAT9000.00153900437.99189421
CAAAGAC13553.3513516E-67.9624041
CGCATAT8150.00563993727.9428641
ATCCCCT8200.00617383657.8632238
CGATTCT8250.006503967.81767464
AACGATA12054.762578E-47.13646652
CAACAAC14901.0018772E-46.7582461
CTTCTAT12809.0932974E-46.74316071
CTCAAAA15301.4156531E-46.58155971
CTATCTG12050.00372166356.54176144
CCATCAT18052.6861519E-56.3758051
CTTTTAA18303.28517E-56.2887041
CTTTGAA18803.1988943E-45.738861
CTTCAAA21957.545857E-55.57065961
CTTCTTT35205.633592E-85.31279331
CTTTTAT24557.164653E-55.27367351
CATAAAT18450.0092602765.06803041
Produced by FastQC (version 0.11.5)