FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504975
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT2502.6666385E-928.7612081
TCCCTAC2003.5538687E-728.7042479
TTCCCTA2156.687096E-726.7016268
CACTCTT3503.9035513E-922.5667462
CTCGACT1950.007169984318.4366721
ACTCTTT5554.5038178E-815.520423
CTTTCCC6003.511281E-410.7672926
CCCCCCC1000.009544237510.382672125-129
CTCTTTC8205.8734324E-59.6292864
TTTCCCT7502.5564304E-49.5680837
CTTTATT7904.219283E-49.1016471
TCTTTCC9001.620765E-48.7733495
Produced by FastQC (version 0.11.5)