FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043284
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGT7101.4551915E-1116.2649121
GCTTAAG8254.2064312E-711.37311651
GTCTAAT6606.645561E-510.9356891
GCTCAGA12257.006747E-910.0161981
GCTAAAT11653.4408004E-89.9125211
GTTTAAT22050.09.8198021
GTAGCAC7452.2631076E-49.6879921
GTTGGGT7400.00228842818.7781081
GCCCAAC8306.6730287E-48.6958481
GTCTAAA8306.6730287E-48.6958481
GCCAGAC12601.1299453E-68.5923271
GCCTCAG9502.7534307E-48.3571681
GCTCAAA21807.2759576E-128.2770121
GTTTAGA8750.00112704028.2486331
GTTAGAA9653.260281E-48.2272631
GCCCAGG9803.8492636E-48.10133651
GTCACAA10801.2409664E-48.0195051
GTTTGAT25051.8189894E-127.77940031
GCTTATC23453.6379788E-117.6946211
GCTTACT10457.660689E-47.5974261
Produced by FastQC (version 0.11.5)