FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535176
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6400.024.0163617
CACGTCT7400.023.6033029
ACACGTC7300.021.055448
GCACACG9350.016.4390076
AGCACAC10850.014.8102575
GAGCACA12000.013.9731554
AGAGCAC12750.013.1512053
GAAGAGC20803.1650416E-107.7680481
CGATCTT13856.9177913E-67.5666914
AAGAGCA23757.730705E-107.06127072
TCGGCCG10950.00177721767.01848037
TCGATCT14809.604481E-46.13685853
GATTTAC14300.00438745235.86286265
CTTTAAC14950.0067573785.63880971
GTCAAAA19400.00394580845.06961
Produced by FastQC (version 0.11.5)