FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6007292
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT31800.07.7856411
CTTCAAT100150.07.05282931
CTCACAT24003.0322553E-96.9784531
CTCAAAT52300.06.82239681
CCCTCAG16303.8127262E-56.70110231
CTTCGAT68350.06.39227251
CTTAGGT12850.00616705656.2335021
CCCAAAT44650.06.19733141
CTCAAAG56950.05.88175151
CCCACTA15100.00507805125.7869121
CCCTGCT56850.05.76400851
CCCCTCT21906.0713908E-55.6525891
CCCCAGG21956.285161E-55.63971231
CTCTAAT21956.285161E-55.63971231
CTCAAGT22006.505852E-55.6268951
CCCAGCT44151.0913936E-115.60777761
CCCACTT35257.6452125E-95.57759761
CTCAAAC45855.456968E-125.55867531
CTCAGAC19654.939964E-45.5586751
CTTACAT35408.44193E-95.5539641
Produced by FastQC (version 0.11.5)