FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501337
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT11452.5387635E-810.1082331
CCCAGTT10701.1129996E-69.4646611
CCCACAC8609.119713E-59.2524141
TGCCTAC6250.0057344679.2283069
CCCTTAT8407.3492614E-48.6115531
CTTCAAT21453.6379788E-128.4308911
CTTCGAG7800.0036105678.3465831
CTCATTT16701.3833414E-88.2299631
CTCATAT13402.6399375E-68.0974311
CTCATTG9853.967415E-48.0782491
CCCATTG9650.0028893157.4960671
CCCAAAA16659.53687E-77.38576461
CTTTCAT24205.475158E-107.17392251
AACCATC12205.116334E-47.09236765
CTCATCT12255.168506E-47.0860781
CACACAG10250.0052031147.05727341
CCCATTT15552.162953E-56.97785041
CCCATCT11500.0020655936.9191961
CCCAGCA20354.4726949E-76.75382761
CTCTGAT10750.0082521156.72902731
Produced by FastQC (version 0.11.5)