FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473724
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5350.032.1902287
CACGTCT7400.024.242369
ACACGTC7200.023.9191278
AGAGCAC9450.019.7427713
GAGCACA10750.018.0246184
AGCACAC11150.016.7326625
GCACACG11950.016.2129656
GAAGAGC19000.09.84548951
AAGAGCA20650.09.3861432
AGTCACA5800.08.41207820-24
TAAATAT14605.900286E-46.3893563
TGATTTG13600.00185322496.33217764
GGTCAAG16600.0026668715.63446661
CAGTCAC7951.0550139E-95.415120-24
GTCAAAA17350.0044969245.3909021
CTGAAAT17450.0048116235.36000871
ACAGTCG7803.7125574E-95.33522625-29
TCGCTTA8601.4479156E-95.17310130-34
ACTCCAG9755.4569682E-115.15114815-19
CACAGTC9551.6189006E-105.108871525-29
Produced by FastQC (version 0.11.5)