FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305188
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGT7100.00153284739.1729821
GGGGGGG1953.2019892E-59.004941130-134
GATTTAA12550.0053691676.31623175
CTTTCAT21709.049767E-66.0025971
CTTCGAC13300.0093841395.98504541
CTTTGAT19407.038774E-55.9682181
GTTTCAT16550.00240165075.68423181
CCCCCCC3050.00634798555.67148990-94
CTCGGCC17450.00449410275.39106231
CCTTCAT17650.0051388295.3299731
TTTCCCG18400.0087815835.091377