FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561645
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAACT6607.8585406E-49.8683431
CCCCCAT7400.0022331998.8014951
GATCCGC12355.9576874E-46.9985975
CCCATTC13100.00110439646.62911561
CTTCGGG15401.4245635E-46.5788951
TATTACC14454.974337E-46.4799586
GTTTAAT12300.00416627256.4719211
CTTACAA13650.00175785656.3620081
CTTCCAC21005.3169097E-66.20295861
CCTCGAT14050.00243217526.18088341
CAATCCT14050.0025624766.1521864
CGATAAC13250.0090247216.0078971
CCTTGAT23053.771127E-65.96524521
CCCGAAA19809.455926E-55.84790661
CTTGAAT15450.0070005585.62080341
CAACAAT19354.3559365E-45.609911
CTCAAAA22057.3793344E-55.579381
CATAGCC15750.0091144485.4877897
CATCAAT28454.1171916E-65.3417391
CAAAGCT20600.00100975875.2695031