FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173399
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC9001.3241595E-48.9405581
CCCCGCT6600.0079601838.866671
ATTGACT6650.0094152048.6867292
CTTCGTT10659.0107285E-58.2422561
CTCAAAT9853.516081E-48.1690381
CTTTGAT18951.5188562E-98.1063361
CACAAAC9500.00221980537.7000021
TTAATGT10457.6095434E-47.60153683
ATCTGGG9650.00293647757.4833786
CTTATAT14901.013936E-57.3640961
GTTTAAT10350.00512738257.0676351
CGATCTT10400.00537310967.03365561
CAACAAG14958.396283E-56.85016871
CTTAAAT12050.00300080146.67759561
CTTTTAA20402.7760343E-66.45441341
CTTCAAT18452.893258E-56.34363371
CACAAAG15056.747846E-46.31860641
CCTTCCT12800.0056396436.28632971
CTTTCAC14000.0020697216.2700021
TAATGTT13850.00211917636.25686074
Produced by FastQC (version 0.11.5)