FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6706815
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAC30302.8012437E-106.41326761
CTCTAAT26202.3735993E-86.3180741
CTTAGAG17701.3985988E-46.0992471
CTCAACT26301.6977174E-76.0203971
CCTTAGT13250.0095629335.974961
GTACGAT34503.5777703E-85.423911
CTCGCAT19250.00265239765.23426251
CCCCAAT29006.476568E-65.2117011
CTAAACT22155.088958E-45.1988161
CACACAC23652.3030813E-45.1733991
CTCGCGT18100.0073712935.1691961
CCTCGAT61050.05.06921861
CTCCAAT52802.0008883E-115.043431
CTCAAAT55803.6379788E-125.0302391
TCCCTAC20050.00448006345.01878269
Produced by FastQC (version 0.11.5)