FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945314
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAT4904.6351197E-513.3822191
CTTAGTT4250.003244617512.0002661
CTCAGCT4500.00497283211.3335841
TCGAACT4700.007539896310.7169542
CTCCGCT6506.4137887E-410.0881351
CTTTCGA5850.00304356999.963591
CTCATTT11051.4699253E-79.8903281
CTCAAAG10406.8331065E-79.8079091
CTTATAT16459.094947E-129.7440281
CTCTTAT10608.863408E-79.6228541
CCCATCT6100.00430178559.5552471
CTTCAAA20050.09.4480181
CTCAATT8507.412324E-59.4287811
CAACGAG6200.00491911179.401131
CTCCTGT7853.4762485E-49.281371
CTCCGTT8507.690039E-48.5716191
CTTTCGG10608.930082E-58.2481611
CTTCTCT11653.0221592E-58.1301611
AACTTGT8150.0056015577.94866135
CTCAAAA15101.5631522E-67.72013241
Produced by FastQC (version 0.11.5)