FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661916
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAGC7652.664271E-49.5317471
CTCATAT16054.6907735E-77.72338631
CCTCAAT12807.7894836E-46.83604861
CTTCGAC12300.00384956326.52110861
CCCTCTT12500.004556086.41677141
CTTAAAT15600.00108193626.07648751
CTCAAAG19758.1729886E-55.9072691
CTTCGTT16200.00170085245.85143231
AGAAGTG15250.0062405415.679624
CTTGTTG21252.3404181E-45.49028541
CTTGAAG20056.350369E-45.4552011
CTTTAAT25451.7622158E-55.4437691
TCAGATT21503.1982167E-45.37126162
CTCATAA17650.00470098345.37072041
CTATAAT20659.42803E-45.29669671
CTTCAAT22204.358247E-45.2553411
GTTTTAC19450.00255898395.2485861
GGAAGAG21450.00156385815.09915071
CTTCATT23007.180826E-45.07254651
Produced by FastQC (version 0.11.5)