FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496929
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAAC3153.6779558E-416.0074771
ACCCATA3508.6284825E-414.3067016
CTTAAAT5054.3252385E-614.2640871
TACATGG4000.00769948313.457218145
CTCAAAT4453.3879717E-412.9498681
CTTCGAT5702.11314E-411.3737331
CTTGAAT5902.909499E-410.9881831
GATAAGC6457.0599734E-49.981427
CTCATCA7950.0044420438.1547531
TTTTAAT9854.457934E-47.98851352
TTTAGGT2350.009632816.622242140-144
CTTTTTT19108.695984E-66.41137081
TTTGTTT12900.00768890466.09975622
Produced by FastQC (version 0.11.5)