FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376106
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC4802.4877572E-615.0494919
TTCCCTA5459.2656865E-613.2545988
GGGTACA6600.0088159778.7570226
CTTCAAT20459.949872E-107.78939061
ACGCTCT11500.00204465476.92578461
ACCGATT12657.5661973E-46.85332162
TCCGATC12200.00389746586.5132228
CGCTCTT12250.00406309736.48734472
CTTCCGA15108.167164E-46.21980526
CTTTAAA19851.4674288E-56.20101171
CTTCAAA23204.1704734E-65.9297961
AAACCAA15050.0053348455.7604335
CTTTGAT24601.1216505E-55.59232761
CTTTCAT27351.976723E-65.55950451
CTTGAAA24757.3411145E-55.26588631
TTCAATC18050.00681427355.2032722
CTGAAAT23752.2352772E-45.182741
CAAAGAA38506.661685E-85.07781841
CTTTTGA20250.0046166535.0058421
Produced by FastQC (version 0.11.5)