FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541912
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC6608.693668E-49.7602752
CTCGATT6450.0075274098.92767051
CCCGATT10156.208134E-58.5098731
GTCCAAT8050.00501703338.047381
CGAAGGG8350.0069671527.75825261
CTCATGC9500.00260167627.5767731
CTTCAGT11800.00285384836.7099391
CTACCAT11900.003118166.6535531
CATCGAT12100.00371309776.54357671
CTGATCT12300.00440709766.4371771
CCCTGCT18002.5549702E-56.3981641
CATAAGT12450.00500102576.3596211
CCCGGTT12900.00723299066.13777351
CACAGAT15450.00112545016.0565151
CTTCAAC19256.7996516E-55.98269941
CTTTAAT22903.6981655E-65.97208551
CTACAAT15800.00147239635.92235141
CAACCTT15050.0055586975.73921731
CTTTTGT27604.30804E-75.7374841
CTGCTAT15350.00691505575.627051
Produced by FastQC (version 0.11.5)