FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503252
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCAG1650.002704551421.8196726
CCTTAAG2000.00804268218.0730551
CGTCCGC3750.001382756413.4409195
CCCAAAT3800.001482745613.3169891
GTCAAGA5100.001034691211.3399561
CGACAAA5150.00112269411.2298591
CTTTAAG4650.00672004710.88271
TTTCGCC5400.001724021410.6673967
CTCCTTT10000.0041051967.22922231
Produced by FastQC (version 0.11.5)