FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648121
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT14851.5122168E-78.2896121
CCCATCT11653.2692493E-58.0803311
CTCAATA13151.765213E-57.7092831
GGGCACG8650.009382017.5042736
CTCAAAA23352.4556357E-107.44279581
CTCAGGA10050.00425289527.2051941
CTTCGAC12707.711407E-46.84209731
GATCTCG12708.0691074E-46.81490335
CTTATAA22404.6757123E-86.7886441
CTCAAGC11750.00256289156.7790141
CTCAATC11850.00280166426.7218071
CCCCAAA10900.0093391156.64332061
CTCCTAT10950.00976001756.6129861
CTCTGAT12150.00364145056.55583671
CTTTAAA23401.0719668E-76.49853041
CTTCAAT24654.199501E-86.4627521
CTTTCGA13450.00147814666.46056751
CTTGCTT17107.9866615E-56.3519471
CACAGAT16102.5168605E-46.29671241
CTTAAAG14000.00232102336.20675951
Produced by FastQC (version 0.11.5)