FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781181
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT1100.00965964826.3695951
GTCCAAG3655.9456233E-515.8940021
CTTCGAT7001.7826096E-1015.5392271
CCCCAAT4050.002341324512.5336961
CTCAAAG6453.6839883E-612.3671361
CTCCCAT7101.3259581E-410.2135751
CTCAATG6506.6949526E-410.04072951
TTTAACT7953.897403E-59.997743
CCCAGAA5950.00363991019.7501021
CTCTATT6000.00390055949.6688521
CTCCGTT6050.0041772669.5889431
CTTTAAT12901.5041223E-89.5564231
CCCAGAT6350.0062234149.1359221
CTTCCTT15302.744855E-99.0053021
ACAATGT7300.00199908188.9083213
TCTTAAT8407.4212585E-48.6019512
CTTTGAT14408.9665264E-88.5609631
CTCCATT10206.015969E-58.531341
CTCCTTT16258.090865E-98.4788391
CTTTAAA14651.1832708E-78.414871
Produced by FastQC (version 0.11.5)