FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655537
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2202.3304892E-829.5678838
CACACGT2508.0590326E-826.0197357
CACGTCT2701.6993181E-724.0886739
GCACACG3406.6354914E-821.2579546
AGAGCAC3751.8653009E-719.273883
GAGCACA3451.7921902E-618.8548814
CACCTAA2550.001508364317.193941
CCCCGCT2650.001951078316.545111
AGCACAC4007.3433184E-616.2623375
GGTAGAT4603.974046E-412.7085631
CCCAAAT5650.002223804410.3467941
AAGAGCA7752.9233997E-510.2618082
CTTGCTT7200.00159081859.1342791
CCTCCAT7600.00260022828.6535291
GAAGAGC8250.00545292867.9717351
GAATATC14454.7377287E-46.5044182
AGTCACT3100.00111220396.06305620-24
ATTATGA13150.0084363986.0459886
CTATTCG3100.00731449535.598973825-29
CAGTCAC3500.0047114545.370135320-24
Produced by FastQC (version 0.11.5)