FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950420
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT7700.016.789367
CACGTCT7750.016.681479
ACACGTC8401.4551915E-1114.53523358
GCACACG14451.1714292E-99.4436116
AGAGCAC15002.2846507E-99.1003783
CAGTACT8004.6311293E-49.0201921
AGCACAC15403.464629E-88.394685
GAGCACA16651.2950477E-77.7644494
CATAGAG13750.00197145976.29773431
CACACGG12900.0070482236.15330931
CTCAAAG23152.6829543E-55.6108321
CCAGATT22751.3199987E-45.3686442
CATAAAT21553.3165366E-45.3577011
CTTCAAT25702.4694591E-55.33489941
TCACTCG5551.6886934E-55.183501725-29
TCAAAGG24153.2215888E-45.0574192
Produced by FastQC (version 0.11.5)