FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028957
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTAG1700.002936233821.515381
AATCCTA4500.00531419511.2332455
TAGATTC5150.001134709211.2176663
CCCAACT5300.001294832611.0418581
CTCAAAT7350.00190265168.9574241
CCTCATT8557.833793E-48.5558241
CTCAAAG10406.823402E-58.440651
GATTCAA8850.00125324058.1597425
CATAGAT9200.00161789467.9513371
CTTGATT14054.4110784E-67.8098541
TAGATAT9350.0021541487.7233923
CTTCAAT16154.909507E-77.70024161
CATTGGA8700.0086995827.5674791
CTCAAAA14657.977358E-67.48999641
CCCATTT10859.894363E-47.4163621
TTGATTT27403.6379788E-127.37950473
CAACAGA10950.0010908677.3486331
CATCGAA11250.00145328317.1526691
CATCAAT17652.002651E-67.04583021
AACCGCC11800.0027544636.7321152
Produced by FastQC (version 0.11.5)