FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516131
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAC7700.004017478.2483941
CTTTCGT12359.31293E-57.4283791
CTTGGAT10300.00693296366.85141751
CTCAAAT11950.00400785876.49594641
CCTTCAC16203.8010385E-46.0986081
CCTCAAA15500.00148314955.91874031
CTCCAAA14650.0051344765.7804451
AACCATT16150.0024440995.6760272
CTTCAAT22652.700981E-55.608181
GTTCCAG18250.00173418695.41355851
CATCGAA17350.00564520255.2876361
CTTTGAT24257.9819394E-55.2381561