FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846294
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2552.9196199E-622.5471947
ACACGTC2557.439217E-519.7287948
CACGTCT2800.00314902415.4005389
ACGTTTA4151.9146412E-413.8551184
AGCACAC5307.319808E-613.5594225
GCACACG4352.8554973E-413.2165396
CTCTGAT5900.00358021779.7697951
CGTACGA1850.00156449647.9797435130-134
CCAAGGA8600.0089798997.54035141
ATCAAAT13050.00110952226.6254941
TCCTGCT15600.0083920625.5283987
Produced by FastQC (version 0.11.5)