FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498803
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT1601.5334081E-939.074589
ACACGTC2105.966285E-1033.079018
CACACGT2107.231447E-726.463217
AGCACAC3001.5955826E-518.5242445
GCACACG3553.436313E-617.6110766
GAGCACA3053.7928234E-415.9429984
AGAGCAC3357.733057E-414.5152663
GGTAAAT6200.0071284858.984961
GATTTCA7950.0061558877.8640665
TCAAAAA11850.0043140946.44831283