FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487564
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAT1550.001721020623.5841771
CCCTCTC2500.001315858417.5466271
CTCCCCT2100.00998278617.4073681
CCTTATT5150.001021607711.357041
GTCAAAT5300.001298805711.0356151
CTTATTA5700.002380798310.2611851
TCAGTTC7200.00176929689.0273272
CTTCAAT8104.5852823E-49.0260431
CTTTGAT9251.7875672E-48.6942751
CTTTGAA7900.00367032958.3290951
TTGAAAT10650.0076459676.7803953
CTTCTTC16853.978485E-46.07449961
TTCAAAA15400.00688367155.62742472
Produced by FastQC (version 0.11.5)