FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143758
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT7750.00362988868.3419811
CACACAT9750.00342303517.3675621
CCCCTGT11450.00212462466.9010571
CTGGACT10500.007199666.8250173
CTTCGAA14706.3313247E-46.35264251
GTCGTAA13000.0080047636.07823851
GTCCATT16000.00175439535.836491
CTTCAGT15300.00682461635.63401751
AAGATTC23102.8964125E-55.5841053
ATTTTGG27903.5087342E-65.38905866
ATTTAAG17950.0071007415.18533376
AGAATTC21050.00155143425.10243565
CACCAAT22606.930908E-45.0855731
CAACGAA21400.0018500595.0350741
Produced by FastQC (version 0.11.5)