FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926891
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAT17302.6193447E-108.8666851
CCCACGA10050.00390802467.26811841
CCCTCGT10150.0043038877.19651171
CTCAAAT15301.5044752E-57.16123531
CTCATGG15008.950656E-56.8174961
GTCCGAT13100.00728740366.1335161
CCTCATT14350.00277753856.1082591
AACCTTC16600.00258174055.6501275
CCTCAAT15600.0070302135.6188151
CTTTGTT27401.7417005E-65.59830861
GTTCAAC17100.00317038045.55309771
CTTTAAT24254.629963E-55.4218671
CTCCGGA19150.0020606885.3400751
CTTTCAC18100.00619189865.2462971
CAAATCT19400.00283094965.20654063
CTCAAAG23157.6806557E-45.04843851
Produced by FastQC (version 0.11.5)