FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906823
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT5956.184564E-1116.6756659
CACACGT6051.3897079E-915.3066997
ACACGTC6857.992639E-913.5190548
AGAGCAC8055.7389116E-912.3268533
AGCACAC8601.1641532E-912.3063825
GAGCACA8106.2918843E-912.250764
GCACACG8106.3027983E-912.2494096
CTCGTAT5303.1287862E-511.573406145
CAATCTC10608.715836E-411.573406145
GAAGAGC13851.9329673E-78.1623091
AAGAGCA12854.2864158E-57.2090412
AGTCACA6555.3445183E-76.3007746130-134
GTCACAC7552.6282578E-86.28617130-134
ACCATCC11401.382432E-105.8556256135-139
CCAATCT8154.941668E-55.399887140-144
CACACCA9907.0648657E-65.186255140-144
CAGTCAC7707.032471E-45.080413140-144
AATCTCG6800.00431385645.0337176140-144
Produced by FastQC (version 0.11.5)