FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558764
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT6000.00376184479.7124221
CTCGGAG8205.39337E-48.8833121
CCTAAAG7450.00223697638.7998471
CTCCGAT13352.265775E-68.1846261
CCCTGAT13001.39956865E-57.8446491
CCTCAAT12155.129017E-57.79391961
CTAAAAC8450.0069656487.7584441
CTCAGAT8600.0081532167.62312271
TACGGGC8500.0082019987.61804779
CTCAAAT14759.33704E-67.40777971
CCGAGCT11400.00175003367.0287271
CCCGAAG15902.6784308E-56.8719971
CTTGCAT11700.0023018216.8485031
CTCCAGT10850.0084366036.71365551
CTCTGGA10850.0084366036.71365551
CCCGAAT11000.0096305446.6221061
CTTTAAT20052.2425847E-66.5395361
CTAAAAT15651.6131849E-46.51632171
CTCATAG12350.0040592096.4880551
CTTGAAC15107.3954643E-46.27126651
Produced by FastQC (version 0.11.5)