FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877996
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC6003.8016879E-1016.771439
ACACTCT9201.5130536E-710.9472151
TTCCCTA11402.8257818E-79.4575738
CCCAACT10156.251471E-58.5050841
CCCAGAA12701.2113582E-57.93026641
CCCCCCC1850.00191315927.8186326100-104
GTCCAGT10357.1591046E-47.6456741
CAGGTCT10900.00125068417.2554363
CACTCTT14255.6222583E-57.0633472
TACAGTG12050.00360972536.56143679
GGTGGTT12600.00570003976.28037551
CTTCGAT17851.5770418E-46.04528141
CCCGGAG18252.1346606E-45.91278171
AAGCCAG15200.00630375375.6745448
CGTAAAT17150.00393002535.45308971
CTTTCCC25702.6260752E-55.31517846
CTCCAAT18950.0021874985.3147431
CTTGATT26053.2541764E-55.24697881
GGTGATT18100.0074013075.1668781
GTTGGAA25201.0807348E-45.13848921
Produced by FastQC (version 0.11.5)