FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513393
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGG6455.1739822E-511.2042592
CTGTAAT6000.00342926069.81941
GGAAGAG7150.00138941859.2700621
CTCAAAG6800.009601748.6641771
CAACATT6800.009601748.6641771
CTTGAAG8100.00430690548.1828331
TCAAAAG10250.00523797247.0504852
TTAATAA10400.00603107466.9487953
ATTTAAA12750.005033326.3537291
Produced by FastQC (version 0.11.5)