FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578399
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1602.4091805E-630.7163817
ACACGTC1909.172767E-625.8664268
CACGTCT2202.853799E-522.3391849
GCACACG3006.1940955E-721.0626626
AGAGCAC4805.3966935E-513.1653023
CCCATGG3250.00972668112.9908371
AGCACAC4100.003269253311.986885
CAAAAAC8258.2409446E-48.5109023
ACGTCTG3400.0047234856.19490059
Produced by FastQC (version 0.11.5)