FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697234
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA3050.00537672314.20912552
CTCTCGG3200.007312928313.5644781
CCCAAGA3900.001794617212.9847991
AGATCGG4500.00530251711.2357342
TCGGACT4650.00677870210.8709529
CTTCGAA4800.00846681210.5501491
GTCATTG6506.945789E-410.000325
CTCTGAT5850.00322590129.8931811
CTCAAAG7450.0023776638.739531
CTTTTAA12105.2932664E-57.77248331
CTATATT10558.3922636E-47.53106074
ATTAAAT13451.9582915E-46.99234531
CTTCAAT11600.00225642586.86019561
AGATTTC10850.0091388016.65711744
CTTCATT14203.8035217E-46.62303161
GATTTCC12800.0064287276.2067965
CAAGAAA14400.0032082236.02865651
CTTCTTT25601.2605E-45.08667951
Produced by FastQC (version 0.11.5)