FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893590
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGA2050.00939005817.595446
CTTAAAC5301.04758525E-412.26621
CTTCGAA6556.0913702E-511.0281341
CACTCTC4850.00932268310.4144784
CTCAAAA11202.0273728E-79.6742341
CCCAGAA7703.1227688E-49.3810761
CTTAAAA10055.2227006E-69.3437381
CTTCCAT17554.5474735E-119.055011
TAGAATC6550.0083620718.8131253
CAACGAA8256.223998E-48.7556711
CTCCAAA9954.7106536E-58.7116731
CCTCGTT6700.0099767318.62498951
CTTTTAT17856.002665E-108.4981511
CCCATCT8700.00105487438.3027921
AGGTTTT9854.0701198E-48.0581934
CTTTAAT10507.978565E-47.56740051
CTTATAT12054.3448296E-47.19345571
CATTGAT17301.7810471E-67.09816651
CAACAAT13401.9079522E-47.0078041
TCCATTC12456.3899835E-46.95493563
Produced by FastQC (version 0.11.5)