FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811621
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAT8356.3823114E-59.5581981
CTCAAAT6850.00107770949.5328261
CTCCAAT6100.00445124379.5154631
CTCAAAC6400.0066086649.0694261
AACCGGC6450.0082519498.8272232
CTCAAAG9050.00149065728.0171721
AAAGATC8450.0085712697.57971434
CGCCGTT9850.00342428667.3660311
CAATCAT11200.00151095817.12597751
CTTTTAT13602.1662153E-46.93544341
CTTTCAT12606.8772223E-46.9100391
CAAAGAA20053.2661592E-76.87557461
TGAATTT14154.4534446E-46.53813653
CAATAAA12600.00520650166.33420231
CTGCATT12800.00613328956.235231
CACAAAA12850.0063867556.21096851
CAAGAAG18100.00109756345.612021
CTTCTTT24706.861627E-55.28743841
Produced by FastQC (version 0.11.5)