FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3842254
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAA11753.7043984E-411.577596145
TCCCTAC11402.644852E-47.5124869
CTCAGGT15804.160682E-67.23416851
CCCCAAT16801.3426343E-67.2287851
TATTAGC15452.3906874E-56.9289927
CTCCTAT20801.11893314E-76.8689821
CCTCAAT29251.4370016E-106.59422251
CTCAACT16304.9726277E-56.5739951
CCCATAG14154.2651285E-46.5631541
CTCCAAT29702.0736479E-106.494311
CTCAAAA48000.06.39960151
CCCGGCT36950.06.38006641
CTCGAAG31501.2732926E-106.34999231
CTCCAAA38350.06.3334341
CCCAGTT25952.2688255E-86.33163931
CTTTAAT58950.06.3015191
CCCCCAT13650.00203568356.2802121
CTCCAGA32955.032962E-76.1928716145
CTTTGAA62600.06.16233251
CCCAGCT27951.603621E-86.1341611
Produced by FastQC (version 0.11.5)