FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971625
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAT4400.004336689611.5435221
CTCCCTT6850.00107762519.5333361
CTACAAT9402.2888964E-59.2628861
CTCCCAT8054.6598408E-49.0135741
CTCATTT9753.5213954E-58.9303721
TTGGTTG6500.007749868.895428
CTCAAAT9803.739691E-58.88480951
CTTATAT10901.3795874E-58.6538591
CCTTGAT13051.7278326E-68.3401471
CTCTCCG9550.0025297927.597831
CTCCATT11753.0896332E-47.4103091
CTTTAAT14709.387832E-67.40400841
CTTGTAT9900.00359766267.329221
CGTTGAT10050.00416594057.2198281
GTCTGTT10200.00481218847.1136541
CTTTGAA16355.471369E-67.10060171
CTTCAAT13752.4782127E-46.860151
CAACAAT15201.15888965E-46.68309071
CAGATTT15902.1903765E-46.36386163
CTCAAAA12850.0063866186.21131