FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305952
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG10357.216504E-69.1096571
CCTCCTC12951.6266131E-78.9608521
CTTCCGA12155.9957074E-68.3570081
CTTCAAA23503.45608E-117.71562671
CTCATAC9450.00229287857.6748031
AGCTATG8500.0079107317.64873936
CTCTGTT10507.648486E-47.59805441
TCCCATT10608.812244E-47.49784142
CTCATGC9850.00344099977.3631361
CTCCAAA11500.00200876346.93735461
CCTCAAT12959.44923E-46.72063831
CTCAAAA16605.1795803E-56.5536341
CTTAAAA15601.6367667E-46.50882341
CTAAAAT12300.00407054746.4861441
ATTAAAT19309.258731E-66.38837861
CCTCGAT13750.00186119976.32961941
ATTTGAT17451.0323437E-46.2344031
CCTTTAA12900.0066844596.1844641
CTTTAAT14350.0030055716.06496671
AGATTGC13350.0099324695.95220236
Produced by FastQC (version 0.11.5)