FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086830
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG5500.00445480711.50182145
CTTTAGA7008.38612E-611.4888561
CTCAAGG7852.4462497E-611.1761881
CCCAGTA4800.00783430110.6620061
GGCCTGT5950.0034040089.8300371
GATTTAG7250.00189442428.9618085
CTATGAT11703.055163E-58.1234331
GTCCTTT11502.2100951E-47.62896351
CACAAGA8800.0096832837.47725151
CTTTAAC9850.00318051397.4224261
CTGGGAA10050.00387028587.27471641
ATTGAAT15101.23227765E-57.2626731
AATTCTG12707.9757854E-46.8213245
CCCATTT10750.00744199336.8010141
CTCATTT11900.00264521346.75815061
CTTCCAA13009.008062E-46.74869871
GTTTGTT12900.00614796166.2342631
CTAAAAT14400.00285536756.0925751
TTAATAT23751.0109688E-66.07935523
CTTCAAT16100.00152870885.9033651
Produced by FastQC (version 0.11.5)