FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718656
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA3100.00577248714.0578041
GTCCTTA3300.00873043313.2058151
CTCCCAA5300.001373485510.9633191
CTCATGG5400.001605257310.7602941
CTCATTT8506.8451354E-610.2539271
CCCAGAT5900.00334948149.8484051
CTCCGGT7853.922551E-49.169448
CTTTAAT10651.0149666E-58.8658761
CTTGAAT7400.0021575538.833621
CTTATTT10250.0049937287.0860471
ATTAAAT11650.00226438746.85795551
CTTCTCT10700.00757328756.7880361
CTCTTTT17356.3135603E-45.8607941
CAAAAAG17909.399055E-45.6807141
ATATTCC15700.0088468715.5016646
Produced by FastQC (version 0.11.5)