FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420795
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCG1050.008466097527.0897626
CTCCAAT3700.001339594513.4966881
AGTATTC3200.00818639213.3332425
GCTCCAA3250.00906929813.1281165
CAGTATT4050.002703306712.293654
CTCCGGA4650.007412581710.7393011
CAAATAC5850.00370647369.7268433
AATTCCG6100.00525550179.3237687
CTTTTTT15554.5200068E-88.2579631