FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994407
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCAC6606.442651E-510.9690571
GTTAAGG9653.032961E-69.75281
TCGATCC9703.5107194E-58.9339463
ATCGATC11550.00219585966.87944562
GTCCAAA10650.00748348566.79772571
CTTTGAT30252.3283064E-106.4617721
CTTGGAT12650.0055615626.29528471
CCCAGAT19651.25933675E-56.2632481
CCCAGAC13300.0093484465.9876211
CTCTTGA16050.00165892475.8638321
CTCAAAC14850.0044963615.85016441
CGATCCG16100.00177788665.82994134
TCGATTT20852.049288E-45.541763
CTTAAAT17650.005117155.3322671
CATTGAT31508.6836553E-75.28604131
CCAGCCT18050.0066551235.21410041
CAATTAT23752.2425478E-45.1820141
CCGACAA21050.00134048455.1588441
GTCTGGA21400.00166844745.07447051
CTTTAAT25901.5037534E-45.0313671