FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558176
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1650.050.430667
ACACGTC2150.038.70268
CACGTCT2301.8189894E-1236.1785169
GAGCACA3100.033.5526544
AGCACAC3258.913048E-1125.6032585
GCACACG3258.913048E-1125.6032586
CGATAGA2854.0903737E-420.87375145
AGAGCAC4001.198714E-920.8026493
AAGAGCA8504.5656634E-1013.0538112
GAAGAGC7552.1796222E-711.9600661
CAGTCAC2451.2097118E-57.93149720-24
CTCCAGT3352.5828558E-77.45169515-19
ACGTCTG2500.00105420636.65684710-14
ACTCCAG3102.6994373E-46.3967943145
CCATTCG3953.8957496E-66.3231925-29
TCGTATG4403.4891254E-65.99325840-44
CTCGTAT3254.9225776E-45.97762835-39
AGTCACC3100.00181273035.820695420-24
ATTCGGT4309.553619E-55.485423630-34
CGTCTGA3857.22781E-45.40328510-14
Produced by FastQC (version 0.11.5)