FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518143
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGTA4100.003684952211.7962264
CTTATGA5000.001267064811.0686381
CTTTAAA5050.001376853910.9590481
CTCATTT5200.001757468310.6429211
CACAAAT4550.00792980610.6429211
CACATGG4650.00931669610.41404151
CTTTCAT7905.8526406E-59.6325171
TTACTTT6050.00621622859.1361563
AAAGAGT6100.0066511179.061273
CCTTTCT6200.00752270278.9263211
TTCCACA7906.294857E-48.7441589
ATTTGCC7300.00299703948.5182146
CTTCAAA7450.00356131828.3571931
AATATGC7950.0064618547.8217575
CTCCTTT9400.00344930647.35946661
CTTCTTT13353.930443E-57.25472551
CTTTTTT15052.5420664E-56.89491561
CTTCATC12400.00722222736.1368461
Produced by FastQC (version 0.11.5)