FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824772
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT2000.00824601417.9965976
AAACGAT9352.1029307E-48.5666251
CTTCAAT9753.3060135E-48.2151731
AACGATT7900.0042252938.2014792
CTTTCAG14600.00348212085.9849021
CTCTTTT15950.00143296255.9348711
CAGAAAA20200.00416629675.04667431
Produced by FastQC (version 0.11.5)