FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680983
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCAGC7108.8695197E-712.1561526
CCAACGA4900.00949792110.3877271
AAATAGC6305.4137874E-410.2748416
AATTCAG6050.00446866459.5105975
GATACGC7350.00221722558.8070087
CCCAGCC8050.00457212338.1295251
CTTGAAA9050.00145814328.0347061
CCCCGGA9300.00190730567.81871941
CTTTGAA9450.00223215767.69461251
GAAATAG8450.00779290647.6605335
CAAATAT10507.4272515E-47.61766671
TTTCCAA11950.00327819456.62060988
CTTTTCT14050.00229986526.2104561
TTTTTGT17000.00354099955.50006256
Produced by FastQC (version 0.11.5)