FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397734
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGG11854.0966006E-68.5969491
CTCTGAT10205.415136E-47.84742551
CTTTAAT21802.532579E-87.0096681
CCCAGAA12556.367404E-46.95780371
CTTGTTT19459.920139E-66.36010071
CATAGAT13150.0078818716.0869761
CTCATAT13250.0085244236.04103661
CTTTGGT13350.0092130585.9957851
CTCATCC15350.00613006445.68862771
CAATCAG16500.0024180515.6810614
CTCCAAA15800.0084324875.526611
CTTTATT26656.7709243E-65.46094041
CTTCAAT22701.0528444E-45.44951341
CTCTTTT29951.6802042E-65.34515761
CTTAAAA22003.945916E-45.29214761
CCTGCTT22806.5351056E-45.1064581
AATCTTG18550.0095980425.05269245
Produced by FastQC (version 0.11.5)